Characterization of drug-plasma protein interactions using surface plasmon resonance

Characterization of drug-plasma protein interactions using surface plasmon resonance

Binding to plasma proteins is a key parameter in evaluating candidate compounds during the lead optimization and early ADME phases of the drug development process. Surface plasmon resonance (SPR) technology is ideally suited for the analysis of multiple aspects of drug-plasma protein interactions. Here we describe how a BiacoreTM SPR based system was used to analyze a panel of low molecular wei...

Fabrication and Characterization of the Fiber Optical Taper for a Surface Plasmon Resonance Sensor

For a fiber optical surface plasmon resonance (SPR) sensor a short part of its cladding should be removed to coat a thin layer of a metal. Usually this is problematic when an optical fiber with small core diameter is used. In this paper, a new method using µliter droplet of the HF acid for short fiber optical taper fabrication is reported. Using this method in a multi-mode optical fiber w...

Surface Effects on DNA-Drug Interactions: Characterization of Actinomycin D Binding by Surface Plasmon Resonance

Behenic Acid Monolayer and Bilayer Assemblies- A Study of Concanavalin A (Con A) Adsorption and its Interaction with Dextran Using Surface Plasmon Resonance Spectroscopy and Microscopy

Deposition of behenic acid (B.A) mono and bilayers onto gold coated surfaces was performed by Langmuir-Blodget dip-casting technique. Surface Plasmon resonance (SPR) and ellipsometry methods were employed for investigation of the monolayer and bilayer films. The adsorption of the biologically important molecule Concanavalin A (Con A) from bulk solution to these monolayers and bilayers as we...

Investigating the Effects of Some Capping Agents upon Gold Nanocrystals Stability, Size and Surface Plasmon Resonance